(李玲)
Date of Birth: 1986.11
E-mail: liling1113@zust.edu.cn; liling1113@hanmail.net
Languages Known: Chinese, Korean (TOPIK 6 grade), English

2016.09-2016.11: Institute of Agricultural Science and Technology, Chungbuk National University (Post-doc Scientist)
2016.12-present: Department of Food Science and Technology, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology (assistant professor)

2005-2009: Dept. of Food Science and Biotechnology,
Jilin Agricultural University, China (B.S) (Grade Point Average 4.07; Excellent graduate)
2010-2012: Dept. of Food Science and Biotechnology,
Chungbuk National University (M.S) (Grade Point Average 4.25/4.5)
Advisors: Nam Soo Han
Thesis: Characterization of the major dehydrogenase related to D-lactate acid synthesis in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
2012- 2016: Dept. of Food Science and Biotechnology,
Chungbuk National University (Ph. D) (Grade Point Average 4.5/4.5)
Advisors: Nam Soo Han
Thesis: Biotechnological Application of Lactic Acid Bacteria to Produce Bioactive Compounds: Phenyllactate, Ginsenoside F2 and C-K, and GDP-L-Fucose

2018
1.L. Li, S.J. Lee, Q.P. Yuan, W.T. Im, S.C. Kim, N.S. Han. 2018 Production of bioactive ginsenoside Rg3(S) and compound K using recombinant Lactococcus lactis. Journal of Ginseng Research. 42(2018):412-418. (IF: 4.029)
2.L. Li, S.A. Kim, R.S. Fang, N.S. Han. 2018. Expression of manB gene from Escherichia coli in Lactococcus lactis and characterization its bifunctional enzyme, phosphomannomutase. Journal Microbiology and Biotechnology. 28(8):1293-1298. (IF: 1.975)
2017
3.L. Li, S-A. Kim, J.E. Heo, T-J. Kim, J-H. Seo, N.S. Han. 2017. One-pot synthesis of GDP-L-fucose by a four-enzyme cascade expressed in Lactococcus lactis. Journal of Biotechnology. 264(2017):1-7 (IF: 3.163)
4.S.B. Lim, J.M.R. Tingirikari, Y.W. Kwon, L. Li, G.E. Kim, N.S. Han. 2017. Polyphasic microbial analysis of traditional Korean jeung-pyun sourdough fermented with makgeolli. Journal Microbiology and Biotechnology. 27(2):226-233.
2016
5.L. Li, N.S. Han. 2016. Application of lactic acid bacteria for food biotechnology. Advanced Biotechnology Book Series. (book chapter)
6.Q. Jin§, L. Li§, J.S. Moon, S.K. Cho, Y.J. Kim, S.J. Lee, N.S. Han. 2016. Reduction of D-lactate content in sauerkraut using starter cultures of recombinant Leuconostoc mesenteroides expressing the ldhL gene. Journal of Bioscience and Bioengineering. 121(5): 479-483. (§co-first author) (IF: 1.964; 1389-1723)
7.J.S. Moon, L. Li, J.S. Bang, N.S. Han. 2016. Application of in vitro gut fermentation models to food components: a review. Food Science and Biotechnology. 25(1): 1-7. (IF: 0.699；1226-7708)
8.L. Li, S-Y. Shin, S.J. Lee, J.S. Moon, W.T. Im, N.S. Han. 2016. Production of ginsenoside F2 by using Lactococcus lactis with enhanced expression of β-glucosidase gene from Paenibacillus mucilaginosus. Journal of Agricultural and Food Chemistry. 64(12): 2506-2512. (IF: 2.857; 0021-8561)
9.J.S. Moon, W.H. Joo, L. Li, H.S. Choi, N.S. Han. 2016. In vitro digestion and fermentation of sialyllactose by infant gut microflora. Journal of Functional Foods. 21(2016): 497-506. (IF: 3.973; 1756-4646)
10.Y.J. Son, A.J. Rye, L. Li, N.S. Han, K.J. Jeong. 2016. Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum. Microbial Cell Factories. 15: 12. （IF: 3.744；1475-2859）
11.N.R. Shin, J.S. Moon, S-Y. Shin, L. Li, Y.B. Lee, T-J. Kim, N.S. Han. 2016. Isolation and characterization of human intestinal Enterococcus avium EFEL009 converting rutin to quercetin. Letters in Applied Microbiology. 62(1): 68-74. (IF:1.579；0266-8254）
2015
12.S.K. Cho, S.J. Lee, S-Y. Shin, J.S. Moon, L. Li, W.H. Joo, D-K. Kang, N.S. Han. 2015. Development of bile salt-resistant Leuconostoc citreum by expression of bile salt hydrolase gene. Journal Microbiology and Biotechnology. 25(12): 2100-2105.
(IF: 1.685; 1017-7825)
13.J.S. Moon, S.Y. Shin, H.S. Choi, W.H. Joo, S.K. Cho, L. Li, J.H. Kang, T.J. Kim, N.S. Han 2015. In vitro digestion and fermentation properties of linear sugar-beetarabinan and its oligosaccharides. Carbohydrate Polymers. 131（2015）: 50-56. （IF:4.219; 0144-8617）
14.A.R. Lee, L. Li, S-Y. Shin, J.S. Moon, H-J. Eom, N.S. Han. 2015. Soluble expression of the fucosyltranferase gene from Helicobacter pylori in Escherichia coli by co-expression of molecular chaperones. Microbiology and Biotechnology Letters. 43(3): 212-218.
15.S.K. Cho, S-Y. Shin, S.J. Lee, L. Li, J.S. Moon, D-J. Kim, W-T. Im, N.S. Han. 2015. Simple synthesis of isomaltooligosaccharides during sauerkraut fermentation by addition of Leuconostoc starter and sugars. Food Science and Biotechnology. 24(4): 1443-1446. (IF: 0.699；1226-7708)
16.J.K. Kim, S-Y, Shin, J.S. Moon, L. Li, S.K. Cho, T-J. Kim, and N.S. Han. 2015. Isolation of dextran-hydrolyzing intestinal bacteria and characterization of their dextranolytic activities. Biopolymers. 103(6): 321-327. （IF:2.248；0006-3525）
2014  
17.L. Li, S-Y. Shin, K.W. Lee, and N.S. Han. 2014. Production of natural antimicrobial compound D-phenyllactic acid using Leuconostoc mesenteroides ATCC 8293 whole cells involving highly active D-lactate dehydrogenase. Letters in Applied Microbiology. 59（4）: 404-411. （ IF:1.579； 0266-8254）
18.Q. Jin§, L. Li§, Y.J. Kim, and N.S. Han. 2014. Construction of a dextran-free Leuconsotoc citreum mutant by targeted disruption of the dextransucrase gene. Journal of Applied Microbiology. 117（4）: 1104-1112. (§co-first author) （IF:2.156; 1364-5072）
19.J.E. Kim, H-J. Eom, L. Li, K-S. Yoo, and N.S. Han. 2014. Induction of the acid tolerance response in Leuconostoc mesenteroides ATCC 8293 by pre-adaptation in acidic condition. Food Science and Biotechnology. 23(1): 221-224. (IF: 0.699；1226-7708)
20.H.Y. Choi, L. Li, S.K. Cho, W-H. Lee, J-H. Seo, and N.S. Han. 2014. Soluble production of CMP-Neu5Ac synthetase by co-expression of chaperone proteins in Escherichia coli. Korean Journal of Microbiology and Biotechnology. 42(2): 190-193.
2013
21.D-M. Li, T-T. Hao, L. Li, N.S. Han, Q. Jin. 2013. Diversity analysis of lactic acid bacteria in Chinese sauerkraut by SDS-PAGE. 食品科技. 38(9): 258-261.
22.H-S. Cui, D-M. Li, Y-R. Li, L. Li, N.S. Han, Q. Jin. 2013. Selection and characterization of antimicrobial Leuconostocs. 生物科技. 23(6): 93-96.  
2012
23.L. Li, H-J. Eom, J-M. Park, E-Y. Seo, J-E. Ahn, T-J. Kim, J.H. Kim, and N.S. -Han. 2012. Characterization of the major dehydrogenase related to D-lactic acid synthesis in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293. Enzyme and Microbial Technology. 51(5): 274-279. （IF: 2.624; 0141-0229）
24.D-M. Li, Y-E. Bai, L. Li, N.S. Han, Q. Jin. 2012. Diversity analysis of lactic acid bacteria in kimchi. 食品科技. 37(11): 10-13.

1.N.S. Han, L. Li. A manufacturing method of phenyllactic acid using Leuconostoc mesenteroides (10-1500830/2015-03-03).
2.K.J. Jeong, Y.J. Son, N.S. Han, L. Li. High-copy number shuttle vector for expression in Leuconostoc sp. (10-2016-0046935/2016-04-18)

1.2018 技术进步奖 二等奖 中国乳制品工业协会
2.2018 中国轻工业联合会科学技术进步奖 三等奖
3.2016 Excellent graduate student poster presentation, KoSFoST (Korean Society of Food Science and Technology) International Symposium and Annual Meeting.
4.2015 Excellent graduate student in the poster presentation, the 3rd Annual Research Workshop of BK21 Education and Research Center at Chungbuk National University.
5.2015 Young investigator award, the 6th International Conference on Food Factors.
6.2015 Best oral presentation, International Symposium on Phytochemicals in Medicine and Food.
7.2015 Certificate of scholarship, Brain Korea 21 Center for Bio-Resource Development.
8.2014 Excellent graduate student in research and academic areas, Brain Korea 21 Center for Bio-Resource Development at Chungbuk National University.
9.2013 Excellent lecture, 2013 Jeonju International Fermented Food Expo, Korea
10.2012 Best graduate student in the English oral presentation competition, 8th Annual Research Workshop of BK21 Education and Research Center at Chungbuk National University.
11.2012 Excellent graduate student poster presentation, 9th Annual Research Workshop of BK21 Education and Research Center at Chungbuk National University.

Microbiological techniques:
Isolation (from fermented foods), Cultivation, Molecular characterization, Adapted laboratory evolution
Molecular Biology:
Genomic and plasmid DNA isolation, Primer designing, PCR, Cloning, Expression of cloned gene, Transformation, Preparation of competent cell, Phylogenetic analysis, RT-PCR, Gene knock-out technology, CRISP-Cas9 technology
Protein works:
Protein expression, SDS-PAGE, Purification, Precipitation, Protein characterization, Western bolt
Instrumentation:
Fermenter, Gel Doc, TLC, HPLC, HPAEC, GPC